04529
Nitrilase
recombinant, expressed in E. coli, ≥2.0 U/mg
Synonyme(s) :
Nitrile aminohydrolase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Forme
crystals
powder
Activité spécifique
≥2.0 U/mg
Poids mol.
41 kDa
Température de stockage
2-8°C
Description générale
Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).
Application
Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.
Actions biochimiques/physiologiques
Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.
Conditionnement
Bottomless glass bottle. Contents are inside inserted fused cone.
Définition de l'unité
1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Alena Petříčková et al.
Applied microbiology and biotechnology, 93(4), 1553-1561 (2011-09-06)
Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 U L(-1)
Einar J Stauber et al.
PloS one, 7(4), e35545-e35545 (2012-04-27)
Plants have evolved a variety of mechanisms for dealing with insect herbivory among which chemical defense through secondary metabolites plays a prominent role. Physiological, behavioural and sensorical adaptations to these chemicals provide herbivores with selective advantages allowing them to diversify
Chen-Sheng Zhang et al.
Applied microbiology and biotechnology, 95(1), 91-99 (2012-03-29)
(R)-o-Chloromandelic acid is the key precursor for the synthesis of Clopidogrel®, a best-selling cardiovascular drug. Although nitrilases are often used as an efficient tool in the production of α-hydroxy acids, there is no practical nitrilase specifically developed for (R)-o-chloromandelic acid.
Shivani Jamwal et al.
International journal of biological macromolecules, 131, 117-126 (2019-03-08)
Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for nitrilase immobilization. Nitrilase was immobilized
Joanna E Raczynska et al.
Journal of structural biology, 173(2), 294-302 (2010-11-26)
The nitrilase superfamily is a large and diverse superfamily of enzymes that catalyse the cleavage of various types of carbon-nitrogen bonds using a Cys-Glu-Lys catalytic triad. Thermoactive nitrilase from Pyrococcus abyssi (PaNit) hydrolyses small aliphatic nitriles like fumaro- and malononitryl.
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