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SBR00025

Sigma-Aldrich

Novobiocin Ready Made Solution

100 mg/mL in water

Synonyme(s) :

Novobiocin Ready Made Solution

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About This Item

Formule linéaire :
C31H35N2NaO11
Poids moléculaire :
634.61
Code UNSPSC :
51286504
Nomenclature NACRES :
NA.76

Source biologique

microbial

Pureté

≥90%

Forme

liquid

Concentration

100 mg/mL in water

Couleur

faint yellow to dark brown

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
neoplastics

Mode d’action

DNA synthesis | interferes
enzyme | inhibits

Température de stockage

−20°C

Description générale

Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.

Application

Novobiocin has been used:
  • in cell culture systems to treat mycoplasma contaminations.[3]
  • in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]

Actions biochimiques/physiologiques

Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II

Activity spectrum: Active against gram-positive and gram-negative bacteria

Caractéristiques et avantages

  • Ready available solution reduce the need for preparation time
  • Commonly used in Cell Biology and Biochemical applications
  • High quality antibiotic suitable for mulitple research applications

Notes préparatoires

Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.

Autres remarques

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

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Lot/Batch Number

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Consulter la Bibliothèque de documents

Environmentally constrained mutation and adaptive evolution in Salmonella.
Massey, R. C., Rainey, P. B., Sheehan, B. J., Keane, O. M., & Dorman, C. J.
Current Biology, 9, 1477-1481 (1999)
Biosynthesis of novobiocin by Streptomyces niveus.
Kominek, L. A.
Antimicrobial Agents and Chemotherapy, 1, 123-134 (1972)
Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors.
Kusuma, B. R., Duerfeldt, A. S., & Blagg, B. S.
Bioorganic & Medicinal Chemistry Letters, 21, 7170-7174 (2011)
Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Novobiocin antagonism of amastigotes of Trypanosoma cruzi growing in cell-free medium.
Pate, P. G., Wolfson, J. S., McHugh, G. L., Pan, S. C., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 29, 426-431 (1986)
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