C1484

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

≥95% purity (HPLC), solid

• Synonyme(s) : 1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-, CPM
• Formule empirique (notation de Hill): C₂₄H₂₂N₂O₄
• Numéro CAS: 76877-33-3
• Poids moléculaire : 402.44
• Numéro MDL: MFCD00077334
• Code UNSPSC : 12171500
• ID de substance PubChem : 24892413
• Nomenclature NACRES : NA.47

Propriétés

• product name: 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin, ≥95% (HPLC), solid
• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: solid
• Technique(s): titration: suitable
• Couleur: yellow
• Solubilité: DMSO: soluble; methanol: soluble
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O
• InChI: 1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
• Clé InChI: YGIABALXNBVHBX-UHFFFAOYSA-N

Description

Description générale

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.

Application

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:

• as a nontoxic substitute for conjugation with antibody(93)
• to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)
• in thermostability shift assay of recombinant protein(95)

Actions biochimiques/physiologiques

Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)