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B6529

Sigma-Aldrich

Brilliant Blue R Staining Solution

ethanol solution

Synonyme(s) :

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Formule empirique (notation de Hill):
C45H44N3NaO7S2
Numéro CAS:
6104-59-2
Poids moléculaire :
825.97
Numéro C.I. (Colour Index):
42660
Numéro Beilstein :
5718025
Numéro MDL:
MFCD00041762
Code UNSPSC :
12171500
ID de substance PubChem :
24891914
Nomenclature NACRES :
NA.47

product name

Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)

Forme

liquid

Niveau de qualité

200

Couleur

dark blue

Adéquation

suitable for (for immunoelectrophoresis protein staining)

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

Clé InChI

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

Brilliant Blue R staining solution is especially designed for use in staining protein in agarose gels following immunoelectrophoresis and Ouchterlony gels. The stain contains ethanol and acetic acid so gels do not require fixing prior to staining. The immunoelectrophoresis or Ouchterlony gel is first washed with water and saline to remove non-precipitated proteins and then dried. The gel is then immersed in staining solution for 30 min and destained with 10% acetic acid.
For detection of protein bands following electrophoresis.

Composants

0.5% (w/v) Brilliant Blue R, 45% (v/v) ethanol and 10% (v/v) acetic acid.

Remarque sur l'analyse

Tested for suitability on agarose gels following immunoelectrophoresis.

Informations légales

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Warning

Mentions de danger

H226,H315,H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

81.0 °F - closed cup

Point d'éclair (°C)

27.2 °C - closed cup

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCK1206
SLCC3765
SLBV8912
SLBS3729V
SLBP1995V

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Wei Zhang et al.
International journal of oncology, 54(5), 1719-1733 (2019-03-14)
Ovarian cancer remains the most lethal type of cancer among all gynecological malignancies. The majority of patients are diagnosed with ovarian cancer at the late stages of the disease. Therefore, there exists an imperative need for the development of early
K Kubo
Analytical biochemistry, 213(2), 200-205 (1993-09-01)
In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at
Zhang Xin-Guo Chen Jian-Hua et al.
Journal of chromatographic science, 50(9), 820-825 (2012-06-22)
A human serum albumin and Thymosin α1 (HSA-Tα1) fusion protein was designed and over-expressed in Pichia pastoris. To purify the fusion protein, a new native preparative electrophoresis system that involved a modified device with a sample receiving chamber, and an
Reiner Westermeier
Proteomics, 6 Suppl 2, 61-64 (2006-10-13)
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There
Chun Yi Liau et al.
Journal of bioscience and bioengineering, 106(1), 111-113 (2008-08-12)
A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated
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