318337

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

• Synonyme(s) : 3,6-Bis(dimethylamino)acridine hydrochloride
• Formule empirique (notation de Hill): C₁₇H₁₉N₃ · HCl · xH₂O
• Numéro CAS: 65-61-2
• Poids moléculaire : 301.81 (anhydrous basis)
• Numéro C.I. (Colour Index): 46005
• Numéro Beilstein : 3734978
• Numéro CE : 200-614-0
• Numéro MDL: MFCD00150070
• Code UNSPSC : 12171500
• ID de substance PubChem : 24859123
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 200
• Pureté: ≥98% (HPLC)
• Forme: powder
• Couleur: orange
• Pf: 284-287 °C (lit.)
• Solubilité: H₂O: 0.1%, clear, orange to red
• λmax: 492 nm
• ε (coefficient d'extinction): ≥14,000 at 227-233 nm in water at 0.005 g/L; ≥35000 at 265-271 nm in water at 0.005 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
• Clé InChI: JUAAUEXLEKAEQC-UHFFFAOYSA-N

Description

Application

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:

• to stain intracellular acidic vesicles.
• to measure lysosomal membrane permeability (LMP).
• or autophagy detection.

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Actions biochimiques/physiologiques

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

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Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)