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Key Documents

02538

Millipore

Raka-Ray Agar

suitable for microbiology, NutriSelect® Plus

Synonyme(s) :

Lactic Acid Bacteria Selective Agar Base

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41171609
Nomenclature NACRES :
NA.85

Stérilité

non-sterile

Niveau de qualité

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Composition

N-acetyl glucosamine, 0.5 g/L
agar, 17.0 g/L
betaine hydrochloride, 2.0 g/L
casein enzymic hydrolysate, 20.0 g/L
diammonium hydrogen-citrate, 2.0 g/L
fructose, 5.0 g/L
glucose, 5.0 g/L
liver concentrate, 1.0 g/L
magnesium sulphate, 2.0 g/L
maltose, 10.0 g/L
manganese sulfate, 0.66 g/L
monopotassium phosphate, 2.0 g/L
potassium aspartate, 2.5 g/L
potassium glutamate, 2.5 g/L
yeast extract, 5.0 g/L

Fabricant/nom de marque

NutriSelect® Plus

pH final

5.4±0.2 (25 °C)

Application(s)

agriculture
bioburden testing
food and beverages

microbiology

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Adéquation

Pediococcus spp.
selective for Lactobacillus spp.

Description générale

Raka-Ray Agar was formulated by Saha, Sondag and Middlekauff to monitor the brewing process and analyze it for a wide range of bacteria. The media is found to be superior to several other media for the cultivation of Lactobacilli and Pediococci. It also suppresses the growth of non-lactic acid facultative bacteria that are often associated with lactic beer spoilage.

Application

Raka-Ray Agar is recommended for selective isolation of lactic acid bacteria encountered in beer and the brewing process.

Notes préparatoires

Suspend 38.5 g in 500 ml of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and add contents of 1vial of lactic acid bacteria selective supplement (Cat. No. 14121). Add 3.5 ml of Cycloheximide solution (Cat. No. 18079). Mix well and pour into sterile petri dishes or dispense as desired.

Remarque sur l'analyse

Casein enzymic hydrolysate, liver concentrate, yeast extract provide carbon, nitrogen, amino acids, minerals, vitamins, trace elements and other essential nutrients for growth. Potassium aspartate and potassium glutamate are additional sources of amino acids. Maltose is to detect lactobacilli, which cannot utilize glucose as a carbon source and fructose is the carbon source of Lactobacillus fructivorans. Glucose is needed as the fermentative energy source for the Pediococci. Sorbitan mono-oleate act as a stimulant for lactic acid bacteria in general. Also, N-acetyl glucosamine, liver concentrate yeast extract and betaine hydrochloride are used as growth stimulating agents. Diammonium hydrogen-citrate and Monopotassium phosphate are the buffering agents while Magnesium and Manganese are important trace elements for Lactobacillaceae. The addition of phenylethanol inhibits Gram-negative organisms, while amphotericin B and cycloheximide inhibits growth of yeasts and molds.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

C. Van Keer et al.
Journal of the Institute of Brewing, 89, 361-361 (1983)
European Brewing Convention
Journal of the Institute of Brewing, 87, 303-303 (1981)
Sixty years of Amphotericin B: An Overview of the Main Antifungal Agent Used to Treat Invasive Fungal Infections
Infectious diseases and therapy, 1-33 (2021)

Articles

Culture Method for Beer Quality Control

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

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