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12013819001

Roche

Anti-HA-Peroxidase, High Affinity

from rat IgG1

Synonyme(s) :

antibody

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About This Item

Code UNSPSC :
12352203

Source biologique

rat

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

clone 3F10, monoclonal

Forme

lyophilized (clear, colorless solution after reconstitution)

Conditionnement

pkg of 25 U (25 μg)

Fabricant/nom de marque

Roche

Isotype

IgG1

Séquence de l'épitope

YPYDVPDYA

Température de stockage

2-8°C

Catégories apparentées

Description générale

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.

Spécificité

Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogène

The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

  • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
  • Western blot
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Notes préparatoires

Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 50 mU/ml
  • ELISA: 25 mU/ml
  • Western blot: 50 mU/ml

Reconstitution

Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Asim A Beg et al.
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Jochen Klucken et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(12), 2050-2057 (2006-10-03)
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Michael Röring et al.
The EMBO journal, 31(11), 2629-2647 (2012-04-19)
The dimerisation of Raf kinases involves a central cluster within the kinase domain, the dimer interface (DIF). Yet, the importance of the DIF for the signalling potential of wild-type B-Raf (B-Raf(wt)) and its oncogenic counterparts remains unknown. Here, we show
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The key basic helix-loop-helix (bHLH) transcription factor in iron (Fe) uptake, FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT), is controlled by multiple signaling pathways, important to adjust Fe acquisition to growth and environmental constraints. FIT protein exists in active and inactive

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