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11663925910

Roche

Mycoplasma PCR ELISA

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About This Item

Code UNSPSC :
12352200

Utilisation

sufficient for 96 reactions

Fabricant/nom de marque

Roche

Conditions d'expédition

dry ice

Température de stockage

−20°C

Spécificité

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Application

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.

Caractéristiques et avantages

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
  • High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
  • Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
  • Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
  • Fast results: The entire procedure can be performed in one day
  • Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Conditionnement

1 kit containing 14 components.

Séquence

Chain Length Positive control: 520 bp.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • Lysis Reagent
  • Neutralization Reagent
  • PCR Mix ready-to-use
  • Denaturation Reagent
  • Hybridization Buffer
  • Biotinylated Capture Probe
  • Washing Buffer 10x concentrated
  • Anti-Digoxigenin-POD antibody
  • Conjugate Dilution Buffer
  • TMB Substrate Solution
  • Stop Reagent
  • Positive Control DNA
  • Streptavidin-coated Microplate
  • Adhesive Plate Cover Foils
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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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