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11585614910

Roche

DIG-High Prime DNA Labeling and Detection Starter Kit II

greener alternative

sufficient for 12 labeling reactions (10 ng to 3 μg per assay), sufficient for 24 blots (blots of 100 cm2)

Synonyme(s) :

DIG system, dna labeling

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About This Item

Code UNSPSC :
41105500

Utilisation

sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
sufficient for 24 blots (blots of 100 cm2)

Niveau de qualité

Fabricant/nom de marque

Roche

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Autre catégorie plus écologique

Température de stockage

−20°C

Description générale

DIG-High Prime DNA Labeling and Detection Starter Kit II is a convenient kit for random-primed labeling of DNA templates with digoxigenin (DIG)-11- deoxyuridine triphosphate (dUTP), alkali-labile and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG-High Prime DNA Labeling and Detection Starter Kit II has been used in a variety of hybridization techniques:
  • in Southern blots
  • in northern blots
  • in dot blots
  • in colony and plaque hybridizations
  • for all types of filter hybridization
  • for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

Conditionnement

1 kit containing 7 components.

Principe

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description

  • DIG-High Prime 5x concentrated

  • DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml

  • DNA Dilution Buffer

  • Anti-Digoxigenin-AP Conjugate antibody

  • CSPD ready-to-use

  • Blocking Solution 10x concentrated

  • DIG Easy Hyb Granules

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Jinxue Ruan et al.
Scientific reports, 5, 14253-14253 (2015-09-19)
Transgenic pigs play an important role in producing higher quality food in agriculture and improving human health when used as animal models for various human diseases in biomedicine. Production of transgenic pigs, however, is a lengthy and inefficient process that
Emmanuel Ogwok et al.
Journal of virological methods, 169(2), 296-304 (2010-08-10)
Cassava brown streak disease (CBSD), caused by two distinct species, Cassava brown streak Uganda virus (CBSUV) and Cassava brown streak virus (CBSV), is a major constraint to cassava (Manihot esculenta Crantz) production in Africa. Absence of infectious clones of CBSUV
Rajiv R Mohan et al.
PloS one, 6(4), e18771-e18771 (2011-05-03)
Untargeted and uncontrolled gene delivery is a major cause of gene therapy failure. This study aimed to define efficient and safe tissue-selective targeted gene therapy approaches for delivering genes into keratocytes of the cornea in vivo using a normal or
Yu Du et al.
Tetrahedron, 67(35), 6568-6575 (2011-08-27)
The apoptolidins are 20/21-membered macrolides produced by Nocardiopsis sp. FU40. Several members of this family are potent and remarkably selective inducers of apoptosis in cancer cell lines, likely via a distinct mitochondria associated target. To investigate the biosynthesis of this
Yanrong Liu et al.
Plant & cell physiology, 58(12), 2226-2240 (2017-10-27)
The microRNA393 (miR393) family is one of the conserved miRNA families in the plant kingdom. MiR393 was reported to regulate rice tillering and abiotic stress resistance positively through an auxin signaling pathway. However, little is known about the function of

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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