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11363514910

Roche

DIG Luminescent Detection Kit

greener alternative

sufficient for 50 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization

Synonyme(s) :

luminescent detection kit

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About This Item

Code UNSPSC :
41116134

Pureté

>98% (NMR)

Niveau de qualité

100

Utilisation

sufficient for 50 blots (10 cm x 10 cm each)

Conditionnement

kit of 1 (5 components)

Fabricant/nom de marque

Roche

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

hybridization: suitable

Autre catégorie plus écologique

, Aligned

Température de stockage

−20°C

Description générale

DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Luminescent Detection Kit has been used for highly sensitive and specific detection of DIG-labeled nucleic acids in:
  • critical commercial assays
  • Southern hybridization
  • northern analysis
  • plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.

Conditionnement

1 kit containing 5 components.

Caractéristiques

Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.

Notes préparatoires

Working concentration: 0.5mg/ml for flow cytometry
  • Storage conditions (working solution): Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
  • Blocking Reagent (bottle 4): in solution at 2 to 8°C
  • CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.

Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
  • Immunological detection 1.5 hours
  • Signal detection 5 to 30 minute

Note: Store protected from light.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • DIG-labeled Control DNA 5 µg/ml
  • DNA Dilution Buffer
  • Anti-digoxigenin-AP antibody, Fab fragments 750 U/ml
  • Blocking Reagent
  • CSPD 11.6 mg/ml

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flashNot applicable

Point d'éclair (°C)

does not flashNot applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Circ-ZNF609 is a circular RNA that can be translated and functions in myogenesis.
Legnini I, et al.
Molecular Cell, 66(1), 22-37 (2017)
DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.
Huang D, et al.
Genetics, 203(1), 353-368 (2016)
Dongqing Huang et al.
Genetics, 203(1), 353-368 (2016-03-28)
In response to replication stress, a phospho-signaling cascade is activated and required for coordination of DNA repair and replication of damaged templates (intra-S-phase checkpoint) . How phospho-signaling coordinates the DNA replication stress response is largely unknown. We employed state-of-the-art liquid
Claudia Sigl et al.
Applied and environmental microbiology, 77(3), 972-982 (2010-12-15)
In filamentous fungi, secondary metabolism is often linked with developmental processes such as conidiation. In this study we analyzed the link between secondary metabolism and conidiation in the main industrial producer of the β-lactam antibiotic penicillin, the ascomycete Penicillium chrysogenum.
Jes-Niels Boeckel et al.
Circulation research, 117(10), 884-890 (2015-09-18)
Circular RNAs (circRNAs) are noncoding RNAs generated by back splicing. Back splicing has been considered a rare event, but recent studies suggest that circRNAs are widely expressed. However, the expression, regulation, and function of circRNAs in vascular cells is still
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