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RMHE-120K

Millipore

MILLIPLEX® Rat Metabolic Hormone Expanded Panel - Metabolism Multiplex Assay

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

 kit sufficient for 96 wells

Niveau de qualité

Espèces réactives

rat

Fabricant/nom de marque

Milliplex®

assay range

standard curve range: 1.4-1,000 pg/mL
(TNFα)

standard curve range: 110-80,000 pg/mL
(IL-6)

standard curve range: 110-80,000 pg/mL
(Insulin)

standard curve range: 2.7-2,000 pg/mL
(Glucagon)

standard curve range: 27-20,000 pg/mL
(GLP-1 (active))

standard curve range: 27-20,000 pg/mL
(GLP-1 total)

standard curve range: 4.1-3,000 pg/mL
(GIP (total))

standard curve range: 4.1-3,000 pg/mL
(Ghrelin (active))

standard curve range: 41-30,000 pg/mL
(Amylin (active))

standard curve range: 48-35,000 pg/mL
(Leptin)

standard curve range: 6.9-5,000 pg/mL
(PYY total)

standard curve range: 6.9-5,000 pg/mL
(Pancreatic Polypeptide, PP)

standard curve range: 6.9-5,000 pg/mL
(Secretin)

standard curve range: 69-50,000 pg/mL
(C-Peptide 2)

standard curve range: 69-50,000 pg/mL
(MCP-1/CCL2)

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex® xMAP®)

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

MILLIPLEX® Rat Metabolic Hormone Expanded Panel is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/lysate and culture supernatant samples and rat serum or plasma: Active Amylin, C-peptide 2, Active Ghrelin, Gastric Inhibitory Polypeptide (GIP), Glucagon-like peptide-1 (GLP-1) Active or Total, Glucagon, Interleukin-6 (IL-6), Insulin, Leptin, Monocyte Chemoattractant Protein-1 (MCP-1), Pancreatic Polypeptide (PP), Peptide YY (PYY), Secretin, and Tumor Necrosis Factor alpha (TNFα). Notes: GLP-1 (Active) and GLP-1 (Total) cannot be run together in the same assay. Protease inhibitors should be added upon collection of samples.

Research Category: Metabolism & Endocrinology

Research Sub-Category:

  • Metabolic Syndrome
  • Obesity
  • Diabetes

Application

The MILLIPLEX® Rat Metabolic Hormone Expanded Panel is a bead-based quantitative assay based on the Luminex® xMAP® technology, which enables the simultaneous analysis of up to 14 analytes in a 25 µl sample volume. This is an overnight assay requiring 25 µL rat serum, plasma, or cell/tissue culture supernatants or lysates. No sample dilution is required.

Analytes Available: Amylin (active);C-Peptide 2; Ghrelin (active);GIP (total);GLP-1 (active);GLP-1 (total);Glucagon;IL-6;Insulin;Leptin;MCP-1/CCL2;Pancreatic Polypeptide (PP);PYY (total);Secretin;TNFα.

Cross Reactivity: There was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel.

Sensitivity: Refer to kit protocol for sensitivities of each analyte.

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Conditionnement

96 well plate

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Skull and crossbonesEnvironment

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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