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Key Documents

MABF1472

Sigma-Aldrich

Anti-CD45 (Mouse), redFluor® 710, clone 30-F11 Antibody

clone 30-F11, from rat, redFluor® 710

Synonyme(s) :

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

100

Conjugué

redFluor® 710

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

30-F11, monoclonal

Espèces réactives

mouse

Technique(s)

flow cytometry: suitable

Numéro d'accès UniProt

P06800

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Ptprc(19264)

Description générale

The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Anti-CD45 (Mouse), redFluor® 710, clone 30-F11 Antibody is an antibody against CD45 for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Qualité

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD45 in one million C57Bl/6 splenocytes.

Forme physique

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

redFluor is a registered trademark of Tonbo Biotechnologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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