MABE1122

Anti-HOIP (RNF31) Antibody, clone 1CB2

clone 1CB2, from mouse

• Synonyme(s) : E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 1CB2, monoclonal
• Espèces réactives: human
• Ne doit pas réagir avec: mouse
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_060469
• Numéro d'accès UniProt: Q96EP0
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RNF31(55072)

Description

Description générale

E3 ubiquitin-protein ligase RNF31 (UniProt Q96EP0; also known as HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein) is encoded by the RNF31 (also known as ZIBRA) gene (Gene ID 55072) in human. HOIP is a RING-between-RING (RBR) family E3 ubiquitin ligase and the only enzyme known to catalyze linear-ubiquitin-chain conjugation. HOIP functions as the primary E3 Ub ligase of the immune signaling regulatory complex LUBAC (linear-ubiquitin-chain assembly complex) composed of heme-oxidized IRP2 ubiquitin ligase-1 (HOIL-1L), HOIP, and SHANK-associated RH domain interactor (Sharpin). RBR ligases often exist in autoinhibited conformations and require protein-protein interactions or posttranslational modifications to induce a conformational change for activation. Disruption of HOIP and HOIL-1L interaction in activated B cell-like (ABC) subtype of diffuse large B-cell lymphoma (DLBCL) induces DLBCL death as a result of decreased NF-κB activation due to HOIP inactivation. On the other hand, HOIP K1056R mutant is resistant to inactivation by TLR4 stimulation-induced HOIP K1056 ubiquitination. Human HOIP is a 1072-a.a. protein that contains a polyUb-binding region (a.a. 1-485), a ubiquitin-associated or UBA domain (a.a. 564-615) that mediates HOIL-1L/RBCK1 interactioin, and a C-terminal linear-ubiquitin-chain-determining domain or LDD domain (a.a. 910-1072). In addition, HOIP conatins three RanBP2-type Zinc fingers (a.a. 299-329, 350-379, 409-438), two RING-type Zinc fingers (a.a. 699-747 and 860-909), and one IBR-type Zinc fingers (a.a. 779-841).

Spécificité

Clone 1CB2 detected a ~120 kDa target band only in parental, but not HOIP-knock, Jurkat cells. Clone 1CB2 targets an epitope within the C-terminal LDD domain present in all three human HOIP (RNF31) spliced isoforms reported by UniProt (Q96EP0).

Immunogène

GST-tagged recombinant human HOIP (RNF31) LDD domain.

Application

Detect HOIP using this mouse monoclonal Anti-HOIP (RNF31), clone 1CB2 Antibody, Cat. No. MABE1122, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:10 dilution from a representative lot detected HOIP (RNF31) in human uterus tissue section.

Western Blotting Analysis: 1 µg/mL from a representative lot detected endogenous HOIP (RNF31) in 60 µg of lysate from HEK293T and wild-type, but not HOIP-knockout, Jurkat cells, as well as the overexpression of an myc-tagged human HOIP in transfected HEK293T cells (Courtesy of Professor Kazuhiro Iwai, Kyoto University School of Medicine, Japan).

Western Blotting Analysis: 1 µg/mL from a representative lot detected endogenous HOIP (RNF31) in 60 µg of human (Jurkat, K562, U937), but not mouse (MEF & Bal17.2) cell lysates, as well as the overexpression of an myc-tagged human HOIP in transfected HEK293T cells (Courtesy of Professor Kazuhiro Iwai, Kyoto University School of Medicine, Japan).

Qualité

Immunohistochemistry Analysis: A 1:10 dilution of this antibody detected HOIP in human adrenal gland tissue section.

Description de la cible

~120 kDa observed. 119.7/57.63/102.5 kDa (isoform 1/2/3) calculated. Target band often appears as a doublet with the upper band representing ubiquitinated HOIP. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable