MABC950

Anti-Aminopeptidase N/CD13 Antibody, clone SL13

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Forme d'anticorps: purified antibody
• Niveau de qualité: 100
• Clone: SL13; monoclonal
• Produit purifié par: using protein G
• Espèces réactives: mouse, rat
• Concentration: (Please refer to lot specific datasheet.)
• Technique(s): activity assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès UniProt: P97449

Description

Description générale

Recombinant protein corresponding to mouse Aminopeptidase N/CD13.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Aminopeptidase N/CD13 in mouse kidney cortex, mouse kidney medulla, and mouse colon tissue.
Immunohistochemistry Analysis: A representative lot detected myocardial infarction-/MI-induced infiltration of aminopeptidase N/CD13-positive dendritic cells in murine cardiac tissue (Pereira, F.E., et al. (2013) Cardiovasc. Res. 100(1):74-83).
Western Blotting Analysis: A representative lot detected aminopeptidase N/CD13 upregulation in murine cardiac tissue following myocardial infarction (MI) induction (Pereira, F.E., et al. (2013) Cardiovasc. Res. 100(1):74-83).
Activity Assay Analysis: A representative lot observed Aminopeptidase N/CD13 blocking monocyte trafficking (Subramani, J., et al. (2014). J. Immunol. 191(7):3905-3912).
Activity Assay Analysis: A representative lot observed Aminopeptidase N/CD13 blocks the adhesion to matrigel of WT mouse satellite cells (Rahman. M.M., et al. (2014). Stem Cells. 32(6):1564-1577).
Western Blotting Analysis: A representative lot detected Aminopeptidase N/CD13 in primary muscle satellite cells (Rahman. M.M., et al. (2014). Stem Cells. 32(6):1564-1577).

Qualité

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aminopeptidase N/CD13 in 10 µg of mouse kidney tissue lysate.

Propriétés physiques

~160 kDa observed. The calculated molecular weight is 110 kDa, however Aminopeptidase N/CD13 has been shown as a ~160 kDa band in western blots (Soderberg, et al. (1993). Journal of virology).

Forme physique

Purified rat monoclonal IgG2bκ in buffer containing PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.