MABF2078

Anti-APN/CD13 Antibody, clone DF-5

clone DF-5, from mouse

• Synonyme(s) : EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: DF-5, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG3κ
• Numéro d'accès NCBI: NP_001141
• Numéro d'accès UniProt: P15144
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ANPEP(290)

Description

Description générale

Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967).

Spécificité

Clone DF-5 specifically detects aminopeptidase N in human cells.

Immunogène

Crude cell membrane fraction from adult human papillary and reticular dermal fibroblasts.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in human prostate tissue.

Flow Cytometry Analysis: A representative lot detected APN/CD13 in Flow Cytometry applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).

Immunofluorescence Analysis: A representative lot detected APN/CD13 in Immunofluorescence applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23; Sorrell, J.M., et. al. (2007). Cell Tissue Res. 327(3):499-510).

Western Blotting Analysis: A representative lot detected APN/CD13 in Western Blotting applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in PC3 cell line.

Research Category
Inflammation & Immunology

This mouse monoclonal Anti-APN/CD13, clone DF-5, Cat. No. MABF2078, detects Aminopeptidase N and has been tested for use in Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Qualité

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APN/CD13 in human kidney tissue.

Description de la cible

109.54 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable