MAB4601

Anti-Bax Antibody, NT, aa3-16 hBax protein, clone 2D2

clone 2D2, Chemicon^®, from mouse

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2D2, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NM_004324.3; NM_138761.2; NM_138763.2; NM_138764.2; NM_138765.2
• Numéro d'accès UniProt: Q07812
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... BAX(581)

Description

Description générale

The previously assigned protein identifier Q07814 has been merged into Q07812. Full details can be found on the UniProt database.

Spécificité

Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.

Immunogène

Epitope: N-terminus, aa3-16 hBax protein

Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.

Application

Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Western blot: 1 μg/ml for 2 hours at RT (1:200 dilution)

Immunofluorescence

Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT

(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)

Optimal working dilutions must be determined by end user.

Liaison

Replaces: 04-434

Forme physique

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Stockage et stabilité

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable