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Merck
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Key Documents

CCYTOMAG-90K

Millipore

MILLIPLEX® Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Synonyme(s) :

canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.47

Niveau de qualité

Espèces réactives

canine

Fabricant/nom de marque

Milliplex®

assay range

accuracy: 91-109%
sensitivity: 13.6 pg/mL
(IFN-γ)

sensitivity: 21.0 pg/mL
(MCP-1)

sensitivity: 21.7 pg/mL
(IL-8)

sensitivity: 3.2 pg/mL
(IP-10)

sensitivity: 3.5 pg/mL
(IL-2)

sensitivity: 3.7 pg/mL
(IL-6)

sensitivity: 5.3 pg/mL
(KC-like)

sensitivity: 5.8 pg/mL
(IL-18)

sensitivity: 6.1 pg/mL
(TNFα)

sensitivity: 7.5 pg/mL
(IL-7)

sensitivity: 8.5 pg/mL
(IL-10)

sensitivity: 9.0 pg/mL
(IL-15)

sensitivity: 9.2 pg/mL
(GM-CSF)

standard curve range: 12.2-50,000 pg/mL
(All analytes)

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Conditions d'expédition

wet ice

Description générale

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Spécificité

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
There was no or negligible cross-reactivity among different analytes within the panel.

Application

  • Analytes: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-α.
  • Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
  • Recommended Sample Dilution: 25 μL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant
  • Assay Run Time: Overnight at 4°C or 2 hours at room temperature (20-25° C)
  • Research Category: Inflammation & Immunology

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Conditionnement

Everything you need in a single kit.

Liaison

Replaces: CCYTO-90K

Stockage et stabilité

Recommended storage for kit components is 2 - 8°C

Autres remarques

Please contact Technical Service for linearity of dilution.

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Organes cibles

Respiratory Tract

Code de la classe de stockage

10 - Combustible liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Robert Goggs et al.
Journal of veterinary internal medicine, 36(2), 549-564 (2022-02-02)
Prolonged antimicrobial drug (AMD) treatment is associated with antimicrobial resistance development. Biomarker measurement may aid treatment decision-making. Investigate temporal changes in blood biomarker concentrations in dogs undergoing treatment for pulmonary and intra-abdominal infections; compare time to biomarker concentration normalization with
Bing Wang et al.
Acta biomaterialia, 116, 259-267 (2020-09-17)
In periodontal treatment, topical adjunctive therapy with antimicrobials or anti-inflammatory agents is frequently applied. However, currently available drug carrier biomaterials often exhibit poor perfusion into small crevices, such as the deep and irregular periodontal pockets, due to relatively high viscosity.
Gustavo Rodrigues Martins et al.
Mediators of inflammation, 2016, 9512743-9512743 (2016-03-19)
Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between
Caroline Fredrich Dourado Pinto et al.
PloS one, 17(7), e0271932-e0271932 (2022-07-23)
Dogs with food allergies and enteropathies may require hydrolyzed diets to prevent or reduce clinical signs, therefore the protein sources used in these diets must be previously characterized and evaluated in healthy dogs. This study aimed to investigate the effects
M R Chambers et al.
Journal of translational genetics and genomics, 5(4), 423-442 (2021-01-01)
To molecularly characterize the tumor microenvironment and evaluate immunologic parameters in canine glioma patients before and after treatment with oncolytic human IL-12-expressing herpes simplex virus (M032) and in treatment naïve canine gliomas. We assessed pet dogs with sporadically occurring gliomas

Contenu apparenté

Uncover tips and tricks to multiplexing in this guide from the experts. Learn how you can enhance the power of your research with MILLIPLEX® multiplexing, including industry guidance for immunoassays, multiplexing tips, one-day MILLIPLEX® multiplex assays for limited lab time, and more.

Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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