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CBL221

Sigma-Aldrich

Anti-RNA Polymerase II Antibody, clone ARNA-3

ascites fluid, clone ARNA-3, Chemicon®

Synonyme(s) :

DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

ARNA-3, monoclonal

Espèces réactives

kangaroo, human, Drosophila

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NP_511124.1

Numéro d'accès UniProt

P04052

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... POLR2A(5430)

Description générale

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; UniProt P04052; also known as DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1) is encoded by the RpII215 (also known as CG1554) gene (Gene ID 32100) in drosophila melanogaster (fruit fly). RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Spécificité

ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II in biological materials. Reactive polypeptides after SDS-PAGE: 200 kDa band of nonphosphorylated and 240 kDa band of phosphorylated Pol IIA.
Broad species cross-reactivity expected due to sequence homology.

Immunogène

Epitope: Amino acids 794-822
Purified Drosophila RNA polymerase II

Application

Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.
Western blot
Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
Optimal working dilutions must be determined by the end user.

Forme physique

Supplied as hybridoma culture supernatant concentrate containing 0.09% sodium azide.

Remarque sur l'analyse

Control
Positive for drosophila, kangaroo kidney epithelial cell line PtK2, and human HeLa and HEp-2 cell lines.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Tim Patrick Kaminski et al.
Molecular biology of the cell, 25(14), 2190-2198 (2014-05-23)
Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of
Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster.
Kramer, A, et al.
Molecular & General Genetics, 180, 193-199 (1980)
Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries.
Petersen, G, et al.
Molecular & General Genetics, 249, 425-431 (1995)

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