04-1569

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

clone 4F8, from rat

• Synonyme(s) : DNA-directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4F8, monoclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): ChIP: suitable; ELISA: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_000928
• Numéro d'accès UniProt: P24928
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... POLR2B(5431)

Description

Description générale

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.

Spécificité

This antibody recognizes RNA polymerase II subunit B1.

Immunogène

Epitope: Unknown

Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.

Application

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.

Chromatin Immunoprecipitation Analysis: A representative lot was used by independent laboraotry in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins

Qualité

Evaluated by Western Blot in NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II subunit B1 on 10 µg of NIH/3T3 cell lysates.

Description de la cible

~ 220 kDa

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
NIH/3T3 cell lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable