CBL194F

Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated

clone LP5K, Chemicon^®, from mouse

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: FITC conjugate
• Forme d'anticorps: saturated ammonium sulfate (SAS) precipitated
• Type de produit anticorps: primary antibodies
• Clone: LP5K, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotype: IgG2b
• Numéro d'accès NCBI: NM_005556.3
• Numéro d'accès UniProt: P08729
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KRT7(3855)

Description

Spécificité

The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.

FUSION PARTNER: Sp2/0

Immunogène

Cytoskeletal proteins from cultured HeLa cells

Application

Detect Cytokeratin 7 using this Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated validated for use in IC, IH, IH(P), FC.

Immunohistochemistry: Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application. Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).

Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).

Flow cytometry: 10μL of conjugate per test.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Qualité

Tested

Forme physique

The antibody is ammonium sulphate precipitation followed by ion exchange chromatography. The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.

Stockage et stabilité

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

Autres remarques

feature_concentration_valuePlease refer to lot specific datasheet.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable