CA1026
Anti-Wilm′s Tumor Protein Rabbit pAb
liquid, Calbiochem®
Synonyme(s) :
Anti-WT-1
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
mouse, human, rat
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Isotype
IgG
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... WT1(7490)
Description générale
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm′s tumor protein.
Recognizes the ~47-55 kDa Wilm′s tumor protein in Wilm′s tumor tissue.
This Anti-Wilm′s Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm′s Tumor Protein.
Immunogène
Human
a synthetic peptide corresponding to amino acids from the C-terminus of human Wilm′s tumor protein
Application
Immunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 10 mM PBS, 0.2% BSA, pH 7.6.
Reconstitution
Following initial use, aliquot and freeze (-20°C) for long-term storage.
Remarque sur l'analyse
Positive Control
Wilm′s tumor tissue
Wilm′s tumor tissue
Autres remarques
Huff, V. 1998. Am. J. Med. Genet.79, 260.
Staining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Noortje A M Bax et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 239(8), 2307-2317 (2010-07-27)
Platelet-derived growth factor receptor alpha (Pdgfralpha) identifies cardiac progenitor cells in the posterior part of the second heart field. We aim to elucidate the role of Pdgfralpha in this region. Hearts of Pdgfralpha-deficient mouse embryos (E9.5-E14.5) showed cardiac malformations consisting
Caitlin M Braitsch et al.
Journal of molecular and cellular cardiology, 65, 108-119 (2013-10-22)
During embryonic heart development, the transcription factors Tcf21, Wt1, and Tbx18 regulate activation and differentiation of epicardium-derived cells, including fibroblast lineages. Expression of these epicardial progenitor factors and localization of cardiac fibrosis were examined in mouse models of cardiovascular disease
Noortje A M Bax et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 238(10), 2658-2669 (2009-08-26)
For the establishment of a fully functional septated heart, addition of myocardium from second heart field-derived structures is important. Platelet-derived growth factors (PDGFs) are known for their role in cardiovascular development. In this study, we aim to elucidate this role
Timo H Lüdtke et al.
American journal of physiology. Lung cellular and molecular physiology, 316(5), L767-L783 (2019-02-01)
The mesothelial lining of the lung, the visceral pleura, and of the heart, the epicardium, derive from a common multipotent precursor tissue, the mesothelium of the embryonic thoracic cavity that also contributes to organ-specific mesenchymal cell types. Insight into mesothelial
Parisa Ranjzad et al.
Nephron, 144(10), 509-524 (2020-08-07)
Numerous studies have documented the in vitro differentiation of human pluripotent stem cells (hPSCs) into kidney cells. Fewer studies have followed the fates of such kidney precursor cells (KPCs) inside animals, a more life-like setting. Here, we tested the hypothesis
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