AP200A
Goat Anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate
Chemicon®, from goat
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
goat
Niveau de qualité
Conjugué
alkaline phosphatase conjugate
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Espèces réactives
mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
western blot: suitable
Isotype
IgG
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Description générale
Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Spécificité
Minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins.
Immunogène
Epitope: Kappa light chain
Prepared from purified mouse IgG light chain.
Application
Suggested dilutions:
Western Blotting: 1:5,000 -1:50,000 of the 1 mg/mL stock.
ELISA: 1:5,000 -1:50,000 of the 1 mg/mL stock.
Optimal working dilutions must be determined by the end user.
Western Blotting: 1:5,000 -1:50,000 of the 1 mg/mL stock.
ELISA: 1:5,000 -1:50,000 of the 1 mg/mL stock.
Optimal working dilutions must be determined by the end user.
Goat anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate detects level of Mouse light chain & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Description de la cible
25 kDa
Forme physique
ImmunoAffinity Purified
Lyophilized from a solution in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.Purified by immunoaffinity chromatography.
Stockage et stabilité
Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.4 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Dermal - Aquatic Chronic 3
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
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