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Key Documents

AP184A

Sigma-Aldrich

Donkey Anti-Sheep IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed

0.6 mg/mL, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702

Source biologique

donkey

Niveau de qualité

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

sheep

Fabricant/nom de marque

Chemicon®

Concentration

0.6 mg/mL

Technique(s)

ELISA: suitable
western blot: suitable

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Catégories apparentées

Description générale

Alkaline Phosphatase-conjugated Affinity Purified Donkey anti Goat IgG (H+L)

Spécificité

Based in immunoelectrophresis, this antibody reacts with the heavy and light chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by Elisa and/ or solid phase absorbed to ensure minimal cross reactivity with chicken, guinea pig, Syrian Hamster, horse, human, mouse, rabbit, and rat serum proteins. Antibodies may cross react with immunoglobulins from other species.

Application

1:5,000 - 1:50,000 for Western Blot and Elisa

Optimal working dilutions must be determined by end user.
Donkey anti-Sheep IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed detects level of Donkey Sheep IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Liaison

Replaces: AP305A

Forme physique

ImmunoAffinity Purified
RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Stockage et stabilité

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


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Naoko Sawada et al.
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Oxidized LDL (oxLDL) is a known risk factor for atherogenesis. This study aimed to reveal structural features of oxLDL present in human circulation related to atherosclerosis. When LDL was fractionated on an anion-exchange column, in vivo-oxLDL, detected by the anti-oxidized

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