ABE1943
Anti-HOXB13 Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
Homeobox protein Hox-B13, HOXB13
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... HOXB13(10481)
Description générale
Homeobox protein Hox-B13 (UniProt Q92826) is encoded by the HOXB13 (also known as PSGD) gene (Gene ID 10481) in human. HOXB13 is a homeobox gene family member whose expression is restricted to prostate tissues and is highly correlated with that of androgen receptor (AR). Hox-B13 suppresses androgen-stimulated AR activity via direct AR interaction and thereby inhibits androgen-dependent (AD) prostate cancer (PCa) growth under androgen-rich conditions. On the other hand, HOXB13 is also known to be over-expressed in castration-resistant PCa (CRPC), where it plays a dichotomic role in promoting androgen-independent (AI) PCa growth. The zinc level in human prostate is markedly diminished in prostatic epithelial tumors and continues to decline during progression towards AI growth. HOXB13 over-expression upregulates transcription of its target genes SLC30A10 (ZnT10) and SLC30A4 (ZnT4) for zinc output transporters, which are responsible for the reduction in zinc level in CRPC. The diminished intracellular zinc level in turn stimulates NF-κB signaling and promotes invasion and metastasis of CRPC cells.
Spécificité
This polyclonal antibody selectively reacts with human HOXB13, but not the homologous HOXA13 or HOXD13.
Immunogène
KLH-conjugated linear peptide corresponding to the C-terminus of human HOXB13.
Application
This Anti-HOXB13 Antibody is validated for use in Western Blotting, Immunocytochemistry and Immunohistochemistry for the detection of HOXB13.
Western Blotting Analysis: A representative lot detected HOXB13 expression in human prostate cancer cell lines MDA PCa 2b, LNCaP, and C4-2, but not in prostate cancer DU145, PC3, CWR22Rv-1, or the non-tumorigenic prostate epithelial line P69 (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunocytochemistry Analysis: A representative lot detected HOXB13 nuclear expression in MDA PCa 2b and LNCaP, but not PC3 or DU145, human prostate cancer cell lines (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunohistochemistry Analysis: A representative lot detected differential HOXB13 expressions among various androgen-dependent (AD) and androgen-independent (AI) human prostate cancer tissues (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124; Kim, Y.R., et. al. (2014). Oncogene. 33(37):4558-4567).
Immunocytochemistry Analysis: A representative lot detected HOXB13 nuclear expression in MDA PCa 2b and LNCaP, but not PC3 or DU145, human prostate cancer cell lines (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunohistochemistry Analysis: A representative lot detected differential HOXB13 expressions among various androgen-dependent (AD) and androgen-independent (AI) human prostate cancer tissues (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124; Kim, Y.R., et. al. (2014). Oncogene. 33(37):4558-4567).
Qualité
Evaluated by Western Blotting in LNCaP cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected HOXB13 in 10 µg of LNCaP cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected HOXB13 in 10 µg of LNCaP cell lysate.
Description de la cible
~35 kDa observed
Forme physique
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-HCI, pH 8.0, and 0.1% soidum azide.
Autres remarques
Concentration: Please refer to lot specific datasheet.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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HOXB13 promotes androgen independent growth of LNCaP prostate cancer cells by the activation of E2F signaling.
Kim, YR; Oh, KJ; Park, RY; Xuan, NT; Kang, TW; Kwon, DD; Choi, C; Kim, MS; Nam, KI; Ahn, KY; Jung, C
Molecular Cancer null
Y-R Kim et al.
Oncogene, 33(37), 4558-4567 (2013-10-08)
Characteristically, prostate cancer (PCa) cells exhibit marked decrease in intracellular zinc; however, the mechanism responsible is not clearly understood. HOXB13 is involved in PCa progression and is overexpressed in castration-resistant PCa. DNA microarray analysis of LNCaP Pca cells showed that
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