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Key Documents

71194-M

Millipore

0.5 M THP Solution

Reducing agent compatible with His•Bind chromatography

Synonyme(s) :

Tris(3-hydroxypropyl)phosphine

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About This Item

Code UNSPSC :
41106511

Niveau de qualité

400

Forme

solution

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze

Technique(s)

protein purification: suitable

Application(s)

sample preparation

Température de stockage

−20°C

Catégories apparentées

Description générale

Reducing agent compatible with HIS-BIND® chromatography
THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.

Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.

Caractéristiques et avantages

Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM

Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)

Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione

Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage

Avertissement

Toxicity: Corrosive (L)

Autres remarques

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informations légales

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8B - Non-combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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