648465

Triton^™ X-100 Detergent, Hydrogenated

Nonionic detergent used for the solubilization of membrane-bound proteins.

• Synonyme(s) : Octylphenoxypolyethoxyethanol, Hydrogenated, TRX-100
• Numéro CAS: 92046-34-9
• Code UNSPSC : 12161902
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Forme: viscous liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: colorless
• CMC: 250 μM
• Solubilité: water: 10%
• Conditions d'expédition: ambient
• Température de stockage: 15-25°C
• InChI: 1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3
• Clé InChI: QQJNBKDKLMCALZ-UHFFFAOYSA-N

Description

Description générale

Nonionic detergent used for the solubilization of membrane-bound proteins. Lacks the UV absorbance and fluorescence of TRITON^™ X-100 (Cat. No. 648462), which causes interference in spectophotometric monitoring and assays. Has a critical micelle concentration (CMC) of 250 µM.

Application

• The utilization of Triton X-100 for enhanced two-dimensional liquid-phase proteomics.: This study explores the role of Triton X-100 in enhancing protein separation techniques in biochemistry, particularly emphasizing its application in two-dimensional liquid-phase proteomics to improve resolution and reproducibility (Kim et al., 2011).


• Effects of environment on the photophysical characteristics of mesotetrakis methylpyridiniumyl porphyrin (TMPyP).: Research focuses on the influence of different solvent environments, including those containing Triton X-100, on the photophysical properties of porphyrins, highlighting its utility in enhancing solubility and stability in biochemical assays (Gonccedil;alves et al., 2011).


• Processing of tissue culture cells.: The work describes methods for processing tissue culture cells where Triton X-100 is used to permeabilize cells for various staining procedures, crucial for molecular biology and cellular biochemistry studies (Bratthauer, 2010).

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Harmful (C)

Reconstitution

Following dilution in H₂O, store at room temperature. Diluted stock solutions are stable for up to 3 months at room temperature.

Autres remarques

Fernandez, J., et al. 1992. Anal. Biochem.201, 255.
Tiller, G.E., et al. 1984. Anal. Biochem.141, 262.
Ashani, Y. and Cantravas, G.N. 1980. Anal. Biochem.109, 55.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335,H411
• Conseils de prudence: P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 235.4 °F - closed cup
• Point d'éclair (°C): 113 °C - closed cup