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Merck
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539137

Sigma-Aldrich

Protease Inhibitor Cocktail Set V, EDTA-Free

A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases.

Synonyme(s) :

EDTA free Protease inhibitor

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About This Item

Code UNSPSC :
12352200

Niveau de qualité

Forme

lyophilized

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated (hygroscopic)

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases. Recommended for use with mammalian cell lysates and tissue extracts. Provided as a single vial or a set of 10 vials. When reconstituted each vial contains a 100X stock solutions. When diluted to 1X the cocktail contains the following components:
A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases. Reconstitute each vial with 1 ml H2O to obtain a 100X stock solution. 1X stock solution contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616370), 1 µM E-64 (Cat. No. 324890), and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Supplied with a data sheet.

Spécificité

Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases.

Actions biochimiques/physiologiques

Cell permeable: no
Primary Target
Serine and cysteine proteases, but not metalloproteases
Product does not compete with ATP.
Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Dam. 1 - Skin Corr. 1A

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3


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Keane K Y Lai et al.
Cancers, 13(6) (2021-04-04)
Differential usage of Kat3 coactivators, CBP and p300, by β-catenin is a fundamental regulatory mechanism in stem cell maintenance and initiation of differentiation and repair. Based upon our earlier pharmacologic studies, p300 serine 89 (S89) is critical for controlling differential
Qiongyu Li et al.
Nature protocols, 15(8), 2668-2704 (2020-07-19)
The glycocalyx comprises glycosylated proteins and lipids and fcorms the outermost layer of cells. It is involved in fundamental inter- and intracellular processes, including non-self-cell and self-cell recognition, cell signaling, cellular structure maintenance, and immune protection. Characterization of the glycocalyx
Jonathan L Chen et al.
Journal of the American Chemical Society, 142(19), 8706-8727 (2020-05-05)
Approximately 95% of human genes are alternatively spliced, and aberrant splicing events can cause disease. One pre-mRNA that is alternatively spliced and linked to neurodegenerative diseases is tau (microtubule-associated protein tau), which can cause frontotemporal dementia and parkinsonism linked to

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