524390

Phorbol-12,13-dibutyrate

Strong irritant for mouse skin, but only moderately active as a tumor promoter.

• Synonyme(s) : Phorbol-12,13-dibutyrate, PDBu, PKC Activator II
• Formule empirique (notation de Hill): C₂₈H₄₀O₈
• Numéro CAS: 37558-16-0
• Poids moléculaire : 504.61
• Numéro MDL: MFCD00036783
• Code UNSPSC : 12352211

Propriétés

• Niveau de qualité: 100
• Pureté: ≥99% (HPLC)
• Forme: solid (crystalline)
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: clear
• Solubilité: DMSO: 10 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1
• Clé InChI: BQJRUJTZSGYBEZ-YVQNUNKESA-N

Description

Description générale

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C (PKC). Stimulates the phosphorylation of Na⁺-K⁺ ATPase, thereby inhibiting its activity. Promotes the expression of iNOS in cultured hepatocytes. PDBu can be diluted to 30 µM in aqueous solutions, so it is commonly used in binding studies or in applications requiring high concentrations. It is easier to remove PDBu than PMA from cells in culture.

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C. Stimulates the phosphorylation of Na⁺,K⁺- ATPase, thereby inhibiting its activity. Promotes the expression of inducible NOS in cultured hepatocytes. Commonly used in binding studies or in applications requiring high concentrations of phorbol compounds.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Na⁺,K⁺- ATPase

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Notes préparatoires

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.

Autres remarques

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427.
Lee, E.J., et al. 1994. Brain Res. 665, 127.
Critz, S.D., et al. 1993. J. Neurochem.60, 1175.
Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611.
Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05,GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H300 + H310 + H330 - H314 - H317 - H334 - H351
• Conseils de prudence: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• Code de la classe de stockage: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable