345797

α1-3,6-Galactosidase, Recombinant, E. coli

Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

• Synonyme(s) : α1-3,6-Galactosidase, Recombinant, E. coli, α1-3,6-D-Galactoside Galactohydrolase
• Numéro CAS: 9025-35-8
• Numéro de classification (Commission des enzymes): 3.2.1.22 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130486
• Code UNSPSC : 12352202

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥30 units/mg protein; ≥85 units/mL
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Activité étrangère: Proteases, none detected
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Recombinant α1-3,6-galactosidase expressed in E. coli. Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. Particularly efficient in removing α-linked galactose under conditions where the pH must be neutral or above, as with live cells.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-α-D-galactopyranoside per min at 25°C, pH 6.5.

Forme physique

In 50 mM sodium phosphate buffer, pH 7.5.

Autres remarques

Prime, S., et al. 1996. J. Chromatogr. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable