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Key Documents

331569

Sigma-Aldrich

Ethidium Bromide Adsorber

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.

Synonyme(s) :

Ethidium Bromide Adsorber

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About This Item

Numéro CAS:
69011-20-7
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.25

Niveau de qualité

200

Forme

suspension

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Couleur

gray-brown

Conditions d'expédition

ambient

Température de stockage

15-25°C

InChI

1S/C10H12.C10H10.C8H8/c2*1-3-9-7-5-6-8-10(9)4-2;1-2-8-6-4-3-5-7-8/h3,5-8H,1,4H2,2H3;3-8H,1-2H2;2-7H,1H2

Clé InChI

NWUYHJFMYQTDRP-UHFFFAOYSA-N

Description générale

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.

Actions biochimiques/physiologiques

Cell permeable: no
Primary Target
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Product does not compete with ATP.
Reversible: no

Avertissement

Toxicity: Irritant (B)

Reconstitution

When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.

Autres remarques

MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103.
Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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