287815

3,4-Dichloroisocoumarin

• Synonyme(s) : 3,4-Dichloroisocoumarin
• Formule empirique (notation de Hill): C₉H₄Cl₂O₂
• Numéro CAS: 51050-59-0
• Poids moléculaire : 215.03
• Numéro MDL: MFCD00036960
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Pureté: ≥99% (elemental analysis)
• Niveau de qualité: 100
• Forme: lyophilized solid
• Puissance: 5-100 μM IC₅₀
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Couleur: white
• Solubilité: DMF: 10 mM; DMSO: 10 mM
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C9H4Cl2O2/c10-7-5-3-1-2-4-6(5)9(12)13-8(7)11/h1-4H
• Clé InChI: SUGXUUGGLDCZKB-UHFFFAOYSA-N

Description

Description générale

A potent irreversible inhibitor of serine proteases. Blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Does not affect thiol proteases and metalloproteases. Also has no activity towards β-lactamases. Effective at concentrations ranging from 5-100 µM.

A potent, irreversible inhibitor of serine proteases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Inhibits granzyme B and blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Does not affect thiol proteases and metalloproteases. Does not exhibit any activity towards β-lactamases.

Effective concentration: 5-100 µM; t_½ = 20 min at pH 7.5.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
serine proteases

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Odake, S., et al. 1991. Biochemistry 30, 2217.
Harper, J.W., et al. 1985. Biochemistry24, 1831.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS06
• Mention d'avertissement: Danger
• Mentions de danger: H301,H315,H319,H335
• Conseils de prudence: P261 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable