17-283
Rac1 Activation Assay Kit
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.
Synonyme(s) :
Activation Assay Kit, Rac1 Activity Kit, Rac1 Assay Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.32
Niveau de qualité
Espèces réactives
human, rat, mouse
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable
affinity binding assay: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Informations sur le gène
human ... RAC1(5879)
Description générale
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades. The RAC1 gene is located on the human chromosome at 7p22.1.
Application
Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.
Composants
Anti-Rac1, clone 23A8 (Cat.# 05-389)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Qualité
Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.
Liaison
Replaces: SGT425
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Julie A Cook et al.
The Journal of biological chemistry, 278(37), 35812-35818 (2003-07-05)
Activation of HIV-1-infected T cells through the T cell receptor and costimulatory molecule CD28 induces proviral transcription; however, the mechanism behind this enhanced virus expression is unknown. Jurkat T cells and primary CD4+ T cells expressing a CD8 alpha/CD28 chimeric
Elizabeth A Pickett et al.
Development (Cambridge, England), 135(3), 589-598 (2008-01-15)
Spina bifida, or failure of the vertebrae to close at the midline, is a common congenital malformation in humans that is often synonymous with neural tube defects (NTDs). However, it is likely that other etiologies exist. Genetic disruption of platelet-derived
Anti-arrhythmic surgery for atrioventricular junctional and atrioventricular re-entrant tachycardia--a report of six cases.
Koo, C C, et al.
Annals of the Academy of Medicine, Singapore, 19, 67-72 (1990)
Michelle E Marchese et al.
Biochemistry, 51(41), 8235-8246 (2012-09-14)
Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for
Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar et al.
Blood null
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique