14-736-M

TAO2 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a MAP3K that doubly phosphorylates & activates the MAP2Ks, MEK3 & MEK6. For use in Kinase Assays.

• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in Sf21 cells
• Pureté: 84% (Coomassie blue staining, SDS-PAGE)
• Activité spécifique: 1562 U/mg
• Poids mol.: M_w 138.3 kDa
• Fabricant/nom de marque: Chemicon^®
• Technique(s): activity assay: suitable (kinase)
• Numéro d'accès NCBI: NM_004783.2; NM_016151.2
• Numéro d'accès UniProt: Q9UL54
• Informations sur le gène: human ... TAOK2(9344)

Description

Description générale

N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates and activates the MAP kinase kinases (MAP2Ks) MEK3 and MEK6.

Product Source: expressed in Sf21 cells

Application

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Actions biochimiques/physiologiques

Protein Target: TAO2

Target Sub-Family: STE

Qualité

Routinely evaluated by phosphorylation of 0.8 mg/ml myelin basic protein (MBP). Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO2.

Définition de l'unité

Specific Activity: where one unit of TOA2 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic per minute at 30°C with a final ATP concentration of 100 μM.

Forme physique

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Ni²⁺/NTA-agarose

Stockage et stabilité

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable