1.01374

Brilliant green (hydrogen sulfate) (C.I. 42040)

for microscopy Certistain^®

• Synonyme(s) : Brilliant green (hydrogen sulfate) (C.I. 42040), Diamond green G, Solid green, Ethyl green
• Formule empirique (notation de Hill): C₂₇H₃₄N₂O₄S
• Numéro CAS: 633-03-4
• Poids moléculaire : 482.63
• Numéro MDL: MFCD00011880
• Code UNSPSC : 41116130
• Numéro de classement CE : 211-190-1

Propriétés

• Niveau de qualité: 400
• Forme: solid
• Puissance: 300-400 mg/kg LD₅₀, oral (Rat)
• Composition: Dye, ≥95% spectrophotometric assay
• pH: 2.5 (25 °C, 10 g/L in H₂O)
• Pf: 210 °C (decomposition)
• Solubilité: 100 g/L
• Masse volumique apparente: 500‑600 kg/m³
• εmax: 2210-2350 at 628-632 nm in 50% ethanol at 0.003 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• InChI: 1S/C27H33N2.H2O4S/c1-5-28(6-2)25-18-14-23(15-19-25)27(22-12-10-9-11-13-22)24-16-20-26(21-17-24)29(7-3)8-4;1-5(2,3)4/h9-21H,5-8H2,1-4H3;(H2,1,2,3,4)/q+1;/p-1
• Clé InChI: NNBFNNNWANBMTI-UHFFFAOYSA-M

Description

Description générale

Brilliant green (hydrogen sulfate) (C.I. 42040) for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution makes target structures in bacteriological specimen materials evaluable for diagnostic purposes. In the area of bacteriology, among others the Gram staining method, the Ziehl-Neelsen staining method, and the spore-staining method according to Rakette are used to yield a differentiated image of pathogens. This can be done either by using eosin Y as a counterstain or by modification according to Wirtz using safranine O solution. By this method, spores are always stained green and the cell elements always red.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Remarque sur l'analyse

Dye content (spectrophotometrically): ≥ 95 %
Identity
- Hydrogen sulfate (as sulfur):
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 628 - 632 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2210 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Informations légales

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302 - H319
• Conseils de prudence: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°C): Not applicable