09-258

Anti-phospho-PAK1 (Ser199/Ser204) Antibody

Upstate^®, from rabbit

• Synonyme(s) : Anti-IDDMSSD, Anti-PAKalpha, Anti-alpha-PAK, Anti-p65-PAK
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (100% immunogen homology), rat (100% immunogen homology)
• Fabricant/nom de marque: Upstate^®
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_002567
• Numéro d'accès UniProt: Q13153
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer199/pSer204)
• Informations sur le gène: human ... PAK1(5058) ; mouse ... Pak1(18479) ; rat ... Pak1(29431)

Description

Description générale

p21-activated kinases (PAK) are Ser/Thr kinases, which are activated by, and are effectors of Rho family of GTPases. As such, they are implicated in Actin polymerization and activation of the stress-activated kinase cascades. PAK1 is phosphorylated by cdk5, resulting in its inhibition. PAK2 can be activated by Caspase-cleavage in addition to p21-binding, implicating it in cytoskeletal changes during apoptosis. PAK3 is expressed at high levels in post-mitotic neurons of the developing post-natal cerebral cortex and hippocampus, and is the mutant gene thought responsible for non-syndromic X-linked mental retardation.

Spécificité

Recognizes PAK1 phosphorylated on Ser199 and Ser204 (human)/Ser198 and Ser203 (mouse). Immunogen sequence is higly homolgous (greater than 90%) with PAK2 and PAK3.

Immunogène

Amino Acids encompassing and including phosphorylated Ser199 and Ser204 of human PAK1. Sequence is highly homologous (greater than 90%) to PAK2 and PAK3.

Epitope: Ser199/Ser204 (Central)

Application

Detect phospho-PAK1 (Ser199/Ser204) using this Anti-phospho-PAK1 (Ser199/Ser204) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Qualité

Routinely evaluated by western blot on untreated and pervanadate-treated Jurkat cell lysates.

Description de la cible

65 kDa

Forme physique

Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol

Double affinity purification over both non-phospho-peptide column followed by phopshorylated peptide column.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable