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Key Documents

07-500

Sigma-Aldrich

Anti-p47-phox (mouse) Antibody

Upstate®, from rabbit

Synonyme(s) :

Cyclin-dependent kinase inhibitor p27, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1)

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

mouse

Fabricant/nom de marque

Upstate®

Technique(s)

western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CDKN1B(1027)

Description générale

Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

Spécificité

Recognizes mouse p47-phox, MW ~47 kDa.

Immunogène

KLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox.

Application

Research Category
Neuroscience
Research Sub Category
Oxidative Stress
This Anti-p47-phox (mouse) Antibody is validated for use in WB for the detection of p47-phox (mouse).

Qualité

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected p47-phox in RIPA lysates from RAW 264.7 cells.

Description de la cible

~47 kDa

Liaison

Replaces: 04-240

Forme physique

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Stockage et stabilité

Stable for 1 year at -20ºC from date of receipt.

Remarque sur l'analyse

Control
RAW 264.7 cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The redox-sensitive cation channel TRPM2 modulates phagocyte ROS production and inflammation.
Anke Di,Xiao-Pei Gao,Feng Qian,Takeshi Kawamura,Jin Han,Claudie Hecquet,Richard D Ye et al.
Nature Immunology null
Janet E L Corry et al.
Journal of applied microbiology, 92(3), 424-432 (2002-03-02)
The prevalence and types of salmonella in broiler chickens during transportation and during slaughter and dressing were studied. This was part of a comprehensive investigation of salmonellas in two UK poultry companies, which aimed to find the origins and mechanisms
Cloning and functional expression of the mouse homologue of p47phox.
S H Jackson et al.
Immunogenetics, 39(4), 272-275 (1994-01-01)
A Wales et al.
Journal of applied microbiology, 101(2), 309-318 (2006-08-03)
To evaluate a semiquantitative technique for the enumeration of Salmonella in the environment of layer flocks and to compare findings with those of a standard qualitative technique. Samples were taken from faeces, floor dust, dust on cages, feeders and egg
PtdIns3P binding to the PX domain of p40phox is a physiological signal in NADPH oxidase activation.
Ellson, C; Davidson, K; Anderson, K; Stephens, LR; Hawkins, PT
The Embo Journal null

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