07-441-AF488

Anti-dimethyl-Histone H3 (Lys9) Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• Synonyme(s) : Histone H3.1t, H3/t, H3t, H3/g, dimethyl-Histone H3 (Lys9), AlexaFluor^™ 488 Conjugate
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), yeast (based on 100% sequence homology), chicken (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable
• Numéro d'accès NCBI: NM_003493
• Numéro d'accès UniProt: Q16695
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: dimethylation (Lys9)
• Informations sur le gène: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Spécificité

Histone H3 dimethylated on lysine 9.

Immunogène

Epitope: H3K9me2

KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.

Application

Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells.
The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Qualité

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.

Description de la cible

~17 kDa observed

Forme physique

Protein A purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable