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16447

Millipore

Glucose Bromcresol Purple Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Tryptone Yeast Extract Agar with Bromocresol Purple

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

Agency

according to ISO 21258-1:2017

Quality Level

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

Agar, 15.0 g/L
Brom cresol purple, 0.015 g/L
Casein enzymic hydrolysate, 10.0 g/L
Glucose, 10.0 g/L
Sodium chloride, 5.0 g/L
Yeast extract, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages

microbiology

suitability

Citrobacter spp.
Neisseria spp.
Proteus spp.
Serratia spp.
Shigella spp.
Streptococcus spp.
nonselective and differential for Bacillus cereus
nonselective and differential for Bacillus spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Salmonella spp.
nonselective and differential for coliforms
nonselective and differential for enterobacteriaceae

Application

Glucose Bromcresol Purple Agar is used for isolation and enumeration of Enterobacteriaceae and Bacillus cereus.

Preparation Note

Suspend 41.52 g in 1000 ml distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

Protocols

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

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