07-500

Anti-p47-phox (mouse) Antibody

Upstate^®, from rabbit

• Synonym(s): Cyclin-dependent kinase inhibitor p27, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_000256
• UniProt accession no.: Q09014
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CDKN1B(1027)

Description

General description

Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

Specificity

Recognizes mouse p47-phox, MW ~47 kDa.

Immunogen

KLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox.

Application

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

This Anti-p47-phox (mouse) Antibody is validated for use in WB for the detection of p47-phox (mouse).

Quality

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected p47-phox in RIPA lysates from RAW 264.7 cells.

Target description

~47 kDa

Linkage

Replaces: 04-240

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
RAW 264.7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1