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Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

ZUSAMMENFASSUNG

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

Essigsäure, glacial, ACS reagent, ≥99.7%

Sigma-Aldrich

Essigsäure, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Magnesiumsulfat, anhydrous, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

2-Butanon, ACS reagent, ≥99.0%

Sigma-Aldrich

Magnesiumsulfat, anhydrous, reagent grade, ≥97%

Sigma-Aldrich

Essigsäure, glacial, ≥99.99% trace metals basis

Sigma-Aldrich

Lithiumbromid, ReagentPlus^®, ≥99%

Sigma-Aldrich

Essigsäure -Lösung, suitable for HPLC

Sigma-Aldrich

Essigsäure, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%

Sigma-Aldrich

2-Butanon, suitable for HPLC, ≥99.7%

Sigma-Aldrich

(+)-Natrium-L-ascorbat, crystalline, ≥98%

Sigma-Aldrich

Essigsäure, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

Sigma-Aldrich

(+)-Natrium-L-ascorbat, BioXtra, ≥99.0% (NT)

Sigma-Aldrich

Di-tert-Butyldicarbonat, ReagentPlus^®, 99%

Sigma-Aldrich

HATU, 97%

Sigma-Aldrich

(+)-Natrium-L-ascorbat, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

Magnesiumsulfat, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

Magnesiumsulfat, BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

Natriumcarbonat Decahydrat, puriss. p.a., ≥99.0% (T)

Sigma-Aldrich

Kupfer(I)-bromid, 98%

Sigma-Aldrich

Lithiumbromid, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

Sigma-Aldrich

Magnesiumsulfat, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)

Sigma-Aldrich

Essigsäure, for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Magnesiumsulfat -Lösung, for molecular biology, 1.00 M±0.04 M

USP

Eisessig, United States Pharmacopeia (USP) Reference Standard

Sigma-Aldrich

2-Butanon, ReagentPlus^®, ≥99%

Sigma-Aldrich

Essigsäure, ≥99.5%, FCC, FG

Sigma-Aldrich

Acetylchlorid, reagent grade, 98%

Sigma-Aldrich

Magnesiumsulfat, anhydrous, free-flowing, Redi-Dri^™, reagent grade, ≥97%

Sigma-Aldrich

Tyramin, ≥98.0%