- An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo.
An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo.
Nature communications (2020-11-29)
Francesca Vallese, Cristina Catoni, Domenico Cieri, Lucia Barazzuol, Omar Ramirez, Valentina Calore, Massimo Bonora, Flavia Giamogante, Paolo Pinton, Marisa Brini, Tito Calì
PMID33247103
ZUSAMMENFASSUNG
Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The lack of tools to finely image inter-organelle proximity hindered our understanding on how these subcellular communication hubs mediate and regulate cell homeostasis. We develop an improved and expanded palette of split-GFP-based contact site sensors (SPLICS) for the detection of single and multiple organelle contact sites within a scalable distance range. We demonstrate their flexibility under physiological conditions and in living organisms.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Proteasehemmer-Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
Sigma-Aldrich
Monoklonales Anti-β-Aktin in Maus hergestellte Antikörper, clone AC-15, ascites fluid