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  • A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

Journal of breath research (2008-09-01)
S Smith, H Burden, R Persad, K Whittington, B de Lacy Costello, N M Ratcliffe, C S Probert
ZUSAMMENFASSUNG

First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed.

MATERIALIEN
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Produktbeschreibung

Supelco
SPME-Faseranordnung Divinylbenzol/Carboxen/Polydimethylsiloxan (DVB/CAR/PDMS), needle size 24 ga, StableFlex
Supelco
SPME-Faseranordnung Divinylbenzol/Carboxen/Polydimethylsiloxan (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler
Supelco
SPME-Faseranordnung Divinylbenzol/Carboxen/Polydimethylsiloxan (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder
Supelco
SPME-Faseranordnung Divinylbenzol/Carboxen/Polydimethylsiloxan (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm
Supelco
ORBO Carboxen-564 Röhrchen, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)
Supelco
SPME-Faseranordnung Divinylbenzol/Carboxen/Polydimethylsiloxan (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler
Supelco
SPME-Faseranordnung Polydimethylsiloxan (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 23 ga
Supelco
SPME-Faseranordnung Polydimethylsiloxan (PDMS), df 100 μm(PDMS, needle size 24 ga, for use with manual holder
Supelco
ORBO 78 HBr auf Carboxen® 564 speziell gereinigt (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea
Supelco
SPME-Faseranordnung Polydimethylsiloxan (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 24 ga
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga
Supelco
Carboxen® Adsorptionsmittel, matrix Carboxen® 1000, 60-80 mesh, bottle of 10 g
Supelco
Carboxen®-1010 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.53 mm, average thickness 30 μm
Supelco
SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler
Supelco
Carboxen®-1006 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.53 mm, average thickness 30 μm
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler
Supelco
SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler
Supelco
SPME-Faseranordnung Polyacrylat (PA), df 85 μm(PA, for use with autosampler, needle size 23 ga
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber
Supelco
SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
Supelco
SPME-Faseranordnung Polyacrylat (PA), df 85 μm(PA, for use with autosampler, needle size 24 ga
Supelco
Carboxen®-1010 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.32 mm, average thickness 15 μm
Supelco
SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler
Supelco
SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with manual holder
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler
Supelco
SPME-Faseranordnung Polydimethylsiloxan (PDMS), df 100 μm(PDMS, for use with manual holder, needle size 23 ga
Supelco
SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga
Supelco
SPME StableFlex Methodenentwicklungs-Kit, for use with manual holder