581324-U

Ascentis^® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(e): Ascentis RP18 HPLC Column
• UNSPSC-Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Eigenschaften

• product name: Ascentis^® C18 HPLC-Säule, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• Materialien: stainless steel column
• Agentur: suitable for USP L1 (Similar to Phenomenex Luna C18)
• Produktlinie: Ascentis^®
• Leistungsmerkmale: endcapped
• Hersteller/Markenname: Ascentis^®
• Verpackung: 1 ea of
• Kennzeichnungsgrad: 25% Carbon loading
• Parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• Methode(n): HPLC: suitable; LC/MS: suitable
• L × ID: 15 cm × 4.6 mm
• Oberflächenbereich: 450 m²/g
• Oberflächendeckung: 3.7 μmol/m²
• Verunreinigungen: <5 ppm metals
• Matrix: fully porous particle; silica gel high purity, spherical
• Aktive Matrixgruppe: C18 (octadecyl) phase
• Partikelgröße: 5 μm
• Porengröße: 100 Å
• operating pH range: 2-8
• Anwendung(en): food and beverages
• Trenntechnik: reversed phase

Beschreibung

Allgemeine Beschreibung

Die Ascentis C18 ist eine äußerst stabile und zuverlässige HPLC-Säule, die eine symmetrische Peak-Form sowie eine ausgezeichnete Retention auch schwieriger Verbindungen ermöglicht.

Anwendung

• Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis^® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022).


• A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis^® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022).


• A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis^® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column′s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022).


• Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis^® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022).


• Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis^® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022).

Leistungsmerkmale und Vorteile

• Ausgezeichnete Retention
• Symmetrische Peak-Form
• Hohe Reproduzierbarkeit
• Vollständig LC-MS-kompatibel

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Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable