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HPLC Analysis of Phenylacetic Acid on Ascentis® C18

HPLC Analysis of Phenylacetic Acid on Ascentis® C18 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® C18 HPLC-Säule

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] 20 mM phosphoric acid; [B] acetonitrile; (75:25, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 215 nm

injection

5 μL

sample

50 μg/mL in water: acetonitrile: methanol (75: 20: 5)

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis C18 for the analysis of phenylacetic acid.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany