V8379

Human Vitronectin

from human plasma, powder, suitable for cell culture

• Synonym(e): Serum spreading factor
• CAS-Nummer: 83380-82-9
• MDL-Nummer: MFCD00132575
• UNSPSC-Code: 12352202
• NACRES: NA.75

Eigenschaften

• product name: Vitronectin from human plasma, lyophilized powder, BioReagent, suitable for cell culture
• Biologische Quelle: human plasma
• Qualitätsniveau: 300
• Produktlinie: BioReagent
• Form: lyophilized powder
• Mol-Gew.: A subunit 75 kDa; B subunit 65 kDa
• Verpackung: pkg of 50 μg
• Methode(n): cell culture | mammalian: suitable
• Oberflächendeckung: 0.1 μg/cm²
• Löslichkeit: water: soluble 0.050 mg/mL, clear, colorless
• UniProt-Hinterlegungsnummer: P04004
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... VTN(7448)

Beschreibung

Anwendung

Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 μg/cm². Optimal conditions for attachment depend on cell line and application.

Biochem./physiol. Wirkung

Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.

Komponenten

Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.

Vorsicht

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

Angaben zur Herstellung

This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable