SRP0706

KAT5 (TIP60), Active

recombinant

• UNSPSC-Code: 12352202

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: 25-35 nmol/min·mg (radiometric)
• Mol-Gew.: ~88 kDa
• Konzentration: 0.1 μg/μL
• Hinterlegungsnummer: NM_006388
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... KAT5(10524)

Beschreibung

Anwendung

Useful for acetyltransferase assay.

Biochem./physiol. Wirkung

Full-length recombinant human KAT5 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. KAT5 or K (lysine) acetyltransferase 5 is a signaling protein that belongs to the MYST family of histone acetyl transferases (HATs). KAT5 was originally isolated as an HIV-1 TAT-interactive protein that plays an important role in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins ⁽¹⁾. KAT5 protein is a histone acetylase that is involved in DNA repair and apoptosis and plays an important role in signal transduction. KAT5 acts as a cofactor of TAT that is involved in the regulation of HIV gene expression. KAT5 is also a co-regulator of transcription factors that either promote or suppress tumorigenesis ⁽²⁾.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable