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SRP0288

Sigma-Aldrich

ATP Citrate Lyase Active human

recombinant, expressed in baculovirus, ≥90% (SDS-PAGE)

Synonym(e):

ACLY, ATP-citrate synthase, ATPCL, CLATP

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About This Item

UNSPSC-Code:
12352204
NACRES:
NA.54

Rekombinant

expressed in baculovirus

Assay

≥90% (SDS-PAGE)

Form

aqueous solution

Spezifische Aktivität

≥1.2 pmol/min-μg

Mol-Gew.

147 kDa

NCBI-Hinterlegungsnummer

NM_001096

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

mouse ... ACLY(47)

Allgemeine Beschreibung

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST tag, MW = 147 kDa, expressed in baculovirus expression system.

Anwendung

Active human ATP citrate lyase is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human ATP citrate lyase has been used in a study to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction, and to identity the active site residues involved. Active human ATP citrate lyase has also been used in a study to analyze tumor metabolism to reveal mitochondrial glucose oxidation in genetically diverse human glioblastomas.

Biochem./physiol. Wirkung

ATP citrate lyase is an enzyme involved in fatty acid synthesis that generates cytosolic acetyl-CoA and oxaloacetate from citrate and CoA. ATP citrate lyase is often upregulated in cancer.

Einheitendefinition

One unit is defined as the amount of enzyme required to convert 1 pmol of ADP to ATP/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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Analysenzertifikate (COA)

Lot/Batch Number
50255100915
5025500915

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Die Dokumentenbibliothek aufrufen

Fabiola Márquez et al.
Critical reviews in food science and nutrition, 52(7), 585-594 (2012-04-26)
Several studies have shown that Garcinia cambogia plays an important role in the regulation of endogenous lipid biosynthesis. This effect is specially attributed to (-)-hydroxycitric acid (HCA) inhibiting the enzyme ATP-dependent citrate lyase, which catalyzes the cleavage of citrate to
Isaac Marin-Valencia et al.
Cell metabolism, 15(6), 827-837 (2012-06-12)
Dysregulated metabolism is a hallmark of cancer cell lines, but little is known about the fate of glucose and other nutrients in tumors growing in their native microenvironment. To study tumor metabolism in vivo, we used an orthotopic mouse model
Fan Fan et al.
Biochemistry, 51(25), 5198-5211 (2012-06-05)
ATP citrate lyase (ACL) catalyzes an ATP-dependent biosynthetic reaction which produces acetyl-coenzyme A and oxaloacetate from citrate and coenzyme A (CoA). Studies were performed with recombinant human ACL to ascertain the nature of the catalytic phosphorylation that initiates the ACL

Artikel

Fatty Acid Synthesis and Metabolism in Cancer Cells

Information on fatty acid synthesis and metabolism in cancer cells. Learn how proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sources or are synthesized by the cell.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.