10354074001

Citrate Lyase (CL)

from Klebsiella pneumoniae (Aerobacter aerogenes)

• Synonym(e): Citrate Lyase (CL)
• EC-Nummer: 4.1.3.6 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204

Eigenschaften

• Biologische Quelle: bacterial (Klebsiella pneumoniae)
• Qualitätsniveau: 100
• Form: lyophilized
• Spezifische Aktivität: ≥0.25 U/mg (At 25 °C with citrate as the substrate.)
• Verpackung: pkg of 120 U
• Hersteller/Markenname: Roche
• Methode(n): activity assay: suitable
• Farbe: light cream
• Optimaler pH-Wert: 8.0-9.0
• Löslichkeit: water: soluble
• NCBI-Hinterlegungsnummer: WP_323133469
• UniProt-Hinterlegungsnummer: P45413
• Anwendung(en): life science and biopharma
• Fremdaktivität: ICDH <0.05% (NAD as coenzyme); NADH oxidase <0.05%
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

Anwendung

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

Qualität

Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each

Physikalische Form

Lyophilizate, stabilized with BSA, sucrose, MgSO₄ and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

Angaben zur Herstellung

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash