SHC004
MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA
shRNA sequence targeting tGFP
Synonym(e):
MISSION® Control Vectors
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About This Item
Qualitätsniveau
Produktlinie
MISSION®
Konzentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
Versandbedingung
dry ice
Lagertemp.
−20°C
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Allgemeine Beschreibung
The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Anwendung
MISSION® pLKO.1-puro TurboGFP™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Rechtliche Hinweise
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
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