SCP0252

Thrombin Substrate, fluorogenic

• Empirische Formel (Hill-System): C₃₁H₄₂N₇O₇
• Molekulargewicht: 624.71
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Assay: ≥95% (HPLC)
• Form: lyophilized
• Zusammensetzung: Peptide Content, ≥80%
• Lagerbedingungen: protect from light
• Lagertemp.: −20°C

Beschreibung

Amino Acid Sequence

BOC-Val-Pro-Arg-AFC

Allgemeine Beschreibung

Thrombin has procoagulant and anticoagulant properties. It also has chemotactic and mitogenic effects. Thrombin triggers the growth of mammalian cells, fibroblasts and macrophage- like tumor cell lines. It regulates brain development. Thrombin plays an important role in hemostasis and thrombosis. It is associated with mast cell degranulation along with the release of histamine and enhances the production of IL-1 (interleukin-1) by activated macrophages. Thrombin stimulates vascular permeability and transendothelial cell migration by neutrophils.

Anwendung

Thrombin is a serine protease involved in the coagulation cascade wherein it converts fibrinogen into fibrin. Thrombin may be detected (assayed) using peptides that contain the VPR sequence such as the fluorogenic substrates Boc-VPR-AFC, Boc-Val-Pro-Arg-7-AMC and the chromogenic substrate Boc-Val-Pro-Arg-pNA. These substrates may also be useful to detect other serine proteases.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable